You have been given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend’s genomic DNA. You prepare the gel by digesting with the following restriction enzymes in the following lanes:
Lane 1: size marker
Lane 2: EcoRI
Lane 3: HindIII
Lane 4: BamHI
Your friend happens to be homozygous for the region you are probing. Does that mean you will only see one band in each lane?